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Maus anti-Human IgG2 (Fc Region) Antikörper (HRP)

WB, ELISA Monoclonal NI 25-1 (HP 6207) IgG1 kappa HRP
Produktnummer ABIN457893
  • Target Alle IgG2 Produkte
    IgG2
    Bindungsspezifität
    • 14
    • 5
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Fc Region
    Reaktivität
    • 23
    • 13
    • 9
    • 7
    • 7
    • 1
    • 1
    Human
    Wirt
    • 24
    • 11
    • 10
    • 10
    • 6
    Maus
    Klonalität
    • 31
    • 30
    Monoklonal
    Konjugat
    • 23
    • 9
    • 9
    • 9
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    HRP
    Applikation
    • 33
    • 29
    • 25
    • 22
    • 15
    • 10
    • 8
    • 7
    • 6
    • 4
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), ELISA
    Spezifität
    The reactivity of the antiserum is restricted to a subclass specific determinant on the Fc portion of the IgG2 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig.
    Produktmerkmale
    Horseradish peroxidase-conjugated purified monoclonal mouse antibody to human IgG2, subclass specific
    Enzyme/IgG protein molar ratio (E/P) approximately 1.7. Enzyme marker Horseradish peroxidase enriched for isoenzyme C (RZ=3.2).
    Aufreinigung
    Purified
    Immunogen
    Highly purified monoclonal IgG2 isolated from pooled human serum.
    Klon
    NI 25-1 (HP 6207)
    Isotyp
    IgG1 kappa
  • Applikationshinweise
    To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting.
    The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:100, in ELISA from 1:100 upwards, in Western blotting from 1:250 upwards. These data should be interpreted as general recommendations only.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.
    Konzentration
    0.4 mg/mL
    Buffer
    Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH 7.2).
    Konservierungsmittel
    Without preservative
    Handhabung
    Avoid repeated freezing and thawing. Dilute only prior to immediate use
    Centrifuge product if not completely clear after standing at room temperature.
    Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
    Lagerung
    4 °C/-20 °C
    Informationen zur Lagerung
    The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.
  • Target
    IgG2
    Abstract
    IgG2 Produkte
    Substanzklasse
    Antibody
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