Maus anti-Human IgG2 (Chain kappa) Antikörper

Produktnummer ABIN457929

Kurzübersicht für Maus anti-Human IgG2 (Chain kappa) Antikörper (ABIN457929)

Target: IgG2

Klonalität: Monoklonal

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Klon: NI 6014 (HP 6014)

Isotyp: IgG1 kappa

Produktdetails

Bindungsspezifität: Chain kappa

Reaktivität: Human

Wirt: Maus

Spezifität: The reactivity of the antiserum is restricted to a subclass specific determinant on the Fab portion of the IgG2 molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. Monoclonal mouse antibody to human IgG2, subclass specific

Aufreinigung: Delipidated, heat inactivated lyophilized stable ascites

Immunogen: Highly purified monoclonal IgG1 kappa isolated from pooled human serum

Anwendungsinformationen

Applikationshinweise: To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG2, and immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Konzentration: 1.0 mg/mL

Buffer: No foreign proteins added.

Konservierungsmittel: Without preservative

Handhabung: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Antigendetails

Target: IgG2

Substanzklasse: Antibody