Maus anti-Human IgG2 Antikörper (FITC)

Produktnummer ABIN457889

Kurzübersicht für Maus anti-Human IgG2 Antikörper (FITC) (ABIN457889)

Target: IgG2

Klonalität: Monoklonal

Konjugat: FITC

Applikation: Western Blotting (WB), ELISA

Klon: NI 6014 (HP 6014)

Isotyp: IgG1 kappa

Produktdetails

Reaktivität: Human

Wirt: Maus

Spezifität: The reactivity of the antiserum is restricted to a subclass specific determinant on the Fab part of the IgG2 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig. Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgG2, subclass specific

Produktmerkmale: Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgG2, subclass specific
Fluorochrome/IgG protein molar ratio (F/P) approximately 1.4.

Aufreinigung: Purified

Immunogen: Highly purified monoclonal IgG2 isolated from human serum.

Anwendungsinformationen

Applikationshinweise: To identify the presence of IgG2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50, in ELISA from 1:200 upwards, in Western blotting from 1:400 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Kommentare:

Excitation/Emission wavelength: 494 nm/514 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Konzentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Konservierungsmittel: Without preservative

Handhabung: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Antigendetails

Target: IgG2

Substanzklasse: Antibody