Ziege anti-Human IgE Antikörper (HRP)

Produktnummer ABIN336563

Kurzübersicht für Ziege anti-Human IgE Antikörper (HRP) (ABIN336563)

Target: IgE

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunoassay (IA)

Produktdetails

Reaktivität: Human

Wirt: Ziege

Aufreinigung: Affinity purified using solid phase Human IgE

Immunogen: Goat antiserum was obtained from animals of US origin and under the care of a registered veterinarian. Country of Origin: Goat antiserum was obtained from animals of US origin and under the care of a registered veterinarian.

Anwendungsinformationen

Applikationshinweise: This conjugate is suitable for all immunoassay applications.
The optimal working dilution should be determined by the investigator. Suggested starting dilution: 1:500-1:5,000 for Immunohistochemistry 1:10,000-1:50,000 for ELISA/Western blot

Kommentare:

HRP: Enzyme derived from the plant horseradish, A small molecule that does not cause steric hindrance in antibody-antigen reactions, Highly sensitive, can detect low levels of antibody and can be used in a sensitive ECL assay, Hydrogen peroxide oxidizes chromogenic substrates such as TMB, ABTS, and OPD to produce a soluble product with a distinct color which can be measured by spectrophotometer, The reaction product has a high color stability after the reaction is stopped. Detection OD of 450 nm

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free. Preservative: 0.1 % (v/v) Kathon CG

Konservierungsmittel: Kathon CG

Handhabung: Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Lagerung: 4 °C/-20 °C

Antigendetails

Target: IgE