Maus anti-Human IgE Antikörper (FITC)
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- Target Alle IgE Produkte
- IgE
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- FITC
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Applikation
- Flow Cytometry (FACS)
- Spezifität
- The mouse monoclonal antibody 4H10 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4G7 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).
- Kreuzreaktivität (Details)
- Human
- Aufreinigung
- Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogen
- Purified human IgE.
- Klon
- 4H10
- Isotyp
- IgG1
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- Applikationshinweise
- Flow cytometry: Recommended dilution: 4 μg/mL.
- Kommentare
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The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 0.1 mg/mL
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
-
Do not freeze.
Avoid prolonged exposure to light. - Lagerung
- 4 °C
- Informationen zur Lagerung
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- Target
- IgE
- Abstract
- IgE Produkte
- Hintergrund
- Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E
- Molekulargewicht
- 180 kDa
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