Maus anti-Human IgE Antikörper (FITC)

Produktnummer ABIN192255

Kurzübersicht für Maus anti-Human IgE Antikörper (FITC) (ABIN192255)

Target: IgE

Klonalität: Monoklonal

Konjugat: FITC

Applikation: Flow Cytometry (FACS)

Klon: BE5

Isotyp: IgG1

Produktdetails

Reaktivität: Human

Wirt: Maus

Verwendungszweck: Anti-Hu IgE FITC

Kreuzreaktivität (Details): Human

Produktmerkmale: The antibody BE5 reacts with human IgE, it recognizes an epitope different from the ones recognized by 4G7.325 and 4H10 antibodies to IgE.

Aufreinigung: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Purified human IgE.

Anwendungsinformationen

Applikationshinweise: Flow cytometry: Recommended dilution: 5 μg/mL.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.1 mg/mL

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do not freeze,Protect from prolonged exposure to light

Lagerung: 4 °C

Informationen zur Lagerung: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Referenzen

Kaulfürst-Soboll, Mertens, Brehler, von Schaewen: "Reduction of cross-reactive carbohydrate determinants in plant foodstuff: elucidation of clinical relevance and implications for allergy diagnosis." in: PLoS ONE, Vol. 6, Issue 3, pp. e17800, (2011) (PubMed).

Antigendetails

Target: IgE

Hintergrund: Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E

Molekulargewicht: 180 kDa

Gen-ID: 35