Kaninchen anti-Human Ig (Chain lambda), (Light Chain) Antikörper

Produktnummer ABIN115330

Produktdetails

Target: Ig

Bindungsspezifität: Chain lambda, Light Chain

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Purified

Immunogen: Lambda light chains isolated from pooled urine of patients with Bence Jones proteinuria

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on formalin-fixed paraffin embedded sections: dilute 1: 250-1: 1000in an ABC method (incubation time: 30 minutes at room temperature). Recommended positive control: Tonsil
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Antigendetails

Target: Ig

Andere Bezeichnung: Immunoglobulin (Ig Produkte)

Hintergrund: All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5 kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L, Lambda, 0.4-4.2 mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).