Kaninchen anti-Human Ig (Chain kappa), (Light Chain) Antikörper

Produktnummer ABIN356485

Produktdetails

Target: Ig

Bindungsspezifität: Chain kappa, Light Chain

Reaktivität: Human, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Aufreinigung: Purified

Immunogen: Purified human Kappa light chain from human Bence Jones proteins.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-FixedParaffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 usingstreptavidinapprox. biotin system or polymer system. FFPE tissue sections require antigen retriever (boiling tissue in 10 mM citrate pH 6.0 for10-20 minutes, followed by cooling at RT for 15-20 minutes). Recommended positive control: Human tonsil.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 2.0 mg/mL

Buffer: PBS, pH 7.4 containing 0.05 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Antigendetails

Target: Ig

Andere Bezeichnung: Immunoglobulin (Ig Produkte)

Hintergrund: Human Kappa chain is a 25 kDa light chain present on all 5 immunoglobulin's (IgA,IgG, IgM, IgD, and IgE) and is also present in free form. Kappa and Lambda light chains consists of a variable region and a constant region and can be easily be differentiated by the antigenic properties of a constant region. The ratio of Kappa to Lambda chain is 70:30, most of them are bound to heavy chains of immunoglobulin.