Ziege anti-Maus IgG (Fc Region) Antikörper

Produktnummer ABIN101852

Hersteller Produkt- Nr.: 210-1103

Produktdetails

Target: IgG

Bindungsspezifität: Fc Region

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Hersteller Produkt- Nr.: 210-1103

Hersteller: Rockland

Verwendungszweck: Mouse IgG Fc Antibody

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG, Mouse IgG F(c) and Mouse Serum. No reaction was observed against Mouse IgG F(ab).

Produktmerkmale: Anti-Mouse IgG Antibody generated in goat detects reactivity to Mouse IgG.

Aufreinigung: Anti-Mouse IgG F(c) Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen: Optional[Immunogen]: Anti-Mouse IgG was produced by repeated immunization with mouse IgG F(c) fragment in goat.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-Mouse IgG F(c) antibody has been assayed against 1.0 μg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 5.0 mL

Konzentration: 10.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

, Preservative:0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Antigendetails

Target: IgG

Abstract: IgG Produkte

Substanzklasse: Antibody

Hintergrund: Receptors bind the Fc portion of mouse IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Anti-Mouse IgG F(c) Antibody is ideal for investigators in Immunology, Cancer, and Microbiology research.