Ziege anti-Maus IgG (F(ab')2 Region) Antikörper (HRP)

Produktnummer ABIN101830

Hersteller Produkt- Nr.: 610-1304

Kurzübersicht für Ziege anti-Maus IgG (F(ab')2 Region) Antikörper (HRP) (ABIN101830)

Target: IgG

Klonalität: Polyklonal

Konjugat: HRP

Applikation: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotyp: IgG

Produktdetails

Bindungsspezifität: F(ab')2 Region

Reaktivität: Maus

Wirt: Ziege

Hersteller Produkt- Nr.: 610-1304

Hersteller: Rockland

Verwendungszweck: Mouse IgG F(ab')2 Antibody Peroxidase Conjugated

Kreuzreaktivität (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Mouse IgG, Mouse IgG F(ab')2 and Mouse Serum.  No reaction was observed against Mouse IgG F(c).

Produktmerkmale: Anti-Mouse IgG F(c) generated in goat is a proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH .

Aufreinigung: Anti-MOUSE IgG F(ab')2 (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Mouse IgG F(ab')2 fragment

Anwendungsinformationen

Applikationshinweise: Application Note: Anti-MOUSE IgG F(ab')2 Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:500 - 1:2,500 Western Blot Dilution: 1:1,000 - 1:5,000 ELISA Dilution: 1:10,000 - 1:50,000 Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Konzentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Haltbarkeit: 12 months

Referenzen

Pühler, Willuda, Puhlmann, Mumberg, Römer-Oberdörfer, Beier: "Generation of a recombinant oncolytic Newcastle disease virus and expression of a full IgG antibody from two transgenes." in: Gene therapy, Vol. 15, Issue 5, pp. 371-83, (2008) (PubMed).

Antigendetails

Target: IgG

Substanzklasse: Antibody

Hintergrund: MOUSE IgG F(ab')2 (GOAT) Antibody is generated in goat detects specifically Mouse IgG F(ab')2 fragment. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays. Anti-Mouse IgG F(ab')2 antibody is ideal for investigators involved in serum protein component research.