Ziege anti-Maus IgG (Heavy & Light Chain) Antikörper
-
- Target Alle IgG Produkte
- IgG
-
Bindungsspezifität
- Heavy & Light Chain
-
Reaktivität
- Maus
-
Wirt
- Ziege
-
Klonalität
- Polyklonal
-
Applikation
- ELISA, Immunohistochemistry (IHC), Western Blotting (WB)
- Hersteller Produkt- Nr.
- 610-101-121
- Hersteller
- Rockland
- Verwendungszweck
- Mouse IgG (H&L) Antibody Pre-adsorbed
- Kreuzreaktivität (Details)
- Minimal crossreactivity against Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins. Specificity was confirmed by ELISA at less than 1 % of target signal.
- Produktmerkmale
- Anti-Mouse IgG + IgM Antibody generated in Goat detects reactivity to Mouse IgG and IgM.
- Aufreinigung
- This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Sterilität
- Sterile filtered
- Immunogen
-
Immunogen: Anti-Mouse IgG whole molecule was produced by repeated immunization with Mouse IgG whole molecule in goat.
Immunogen Type: Native Protein
- Isotyp
- IgG
-
-
- Applikationshinweise
- Application Note: Anti-Mouse IgG has been tested by ELISA and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:20,000 ELISA Dilution: 1:50,000 - 1:100,000 Other: User Optimized
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Liquid
- Konzentration
- 0.95 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
, Preservative:0.01 % (w/v) Sodium Azide - Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
- Haltbarkeit
- 12 months
-
- Target
- IgG
- Abstract
- IgG Produkte
- Substanzklasse
- Antibody
- Hintergrund
- Anti-Mouse IgG whole molecule antibody generated in goat detects specifically Mouse IgG whole molecule . This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.
-