GM-CSF Protein

Produktnummer ABIN987967

GM-CSF-Protein exprimiert in Yeast (Pichia pastoris).

Kurzübersicht für GM-CSF Protein (ABIN987967)

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Biologische Aktivität: Active

Spezies: Human

Quelle: Yeast (Pichia pastoris)

Reinheit: > 97 % by SDS-PAGE

Produktdetails

Produktmerkmale: Resuspend cells in assay media free of h-GM-CSF containing 10 % FBS and 2X Gentamicin. Transfer 100ml culture/well to assay plate (19,500 cells/well, passage # 13). Serial dilute h-GM-CSF in assay media in duplicate. Transfer 100 ml/well serial diluted h-GM-CSF to cells in assay plate. Final assay volume of each well is 200 ml containing 2X Gentamicin and h-GM-CSF. Incubate 70 hours. Add 20 ml of Promega Substrate Cell Titer 96 Aqueous One Solution Reagent to each well. Incubate 37°C and read OD at 490 nm. The ED50 for this effect is 0.06 - 0.09 ng/ml.

Endotoxin-Niveau: level is less than 0.03 ng per myg (0.3 EU/myg) of human GMCSF

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Antigendetails

Target: GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))

Andere Bezeichnung: Granulocyte Macrophage Colony Stimulating Factor

Hintergrund: GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Synonym: rHuGM-CSF 7, hGM-CS. Formulation: Lyophilized from a 0.2 mym filtered solution in 10 mM PB, pH7.0.

Pathways: JAK-STAT Signalweg, Cellular Response to Molecule of Bacterial Origin