Avidin Protein (AVD) (HRP)

Produktnummer ABIN964120

Hersteller Produkt- Nr.: a003-03

Native Avidin-Protein exprimiert in Huhn.

Kurzübersicht für Avidin Protein (AVD) (HRP) (ABIN964120)

Target: Avidin (AVD)

Protein-Typ: Native

Spezies: Hühnerei

Quelle: Huhn

Applikation: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails

Aufreinigungstag / Konjugat: Dieses Avidin Protein ist gelabelt mit HRP.

Hersteller Produkt- Nr.: a003-03

Hersteller: Rockland

Verwendungszweck: Avidin Peroxidase Conjugated

Aufreinigung: Avidin Peroxidase Conjugated was prepared from chromatographically pure avidin isolated from egg white followed by extensive dialysis against the buffer stated above.  Avidin Peroxidase Conjugated assayed by immunoelectrophoresis resulted in a single prec

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: Avidin Peroxidase Conjugated is a useful detection reagent for primary antibodies conjugated to biotin. Avidin Peroxidase Conjugated can be utilized in both Western Blotting and ELISA experiment formats in combination with the proper substrate (TMB-1000

Western Blot Dilution: 1:10,000 - 1:40,000

ELISA Dilution: 1:20,000 - 1:200,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Restore with deionized water (or equivalent) (in 1.0 mL)

Konzentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Konservierungsmittel: Gentamicin sulfate

Vorsichtsmaßnahmen: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Do NOT add Sodium Azide! Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  Avidin Peroxidase Conju Expiration date is one (1) year from date of receipt.

Haltbarkeit: 12 months

Antigendetails

Target: Avidin (AVD)

Andere Bezeichnung: Avidin

Hintergrund:

Synonyms: HRP-Avidin conjugated, Horseradish peroxidase conjugated Avidin, AvD, HRP Avidin Biotin Complex, Avidin Peroxidase Conjugated, Avidin HRP Conjugated

Background: Avidin is a biotin-binding protein found in the oviducts of egg-laying animals (birds, reptiles, and frogs) that gets deposited into the whites of their eggs. Avidin is a tetramer and can bind up to four biotin molecules (Vitamin B7) with one of the grea

UniProt: P02701