FAS Protein

Produktnummer ABIN935361

Dieses Recombinant FAS-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für FAS Protein (ABIN935361)

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 98 % pure

Produktdetails

Sequenz: MRLSSKSVNA QVTDINSKGL ELRKTVTTVE TQNLEGLHHD GQFCHKPCPP GERKARDCTV NGDEPDCVPC QEGKEYTDKA HFSSKCRRCR LCDEGHGLEV EINCTRTQNT KCRCKPNFFC NSTVCEHCDP CTKCEHGIIK ECTLTSNTKC KEEGSRS

Produktmerkmale: Purified recombinant Human Fas Receptor protein
Expression System: E.coli
Bioactivity: The ED50 was determined by its ability to inhibit the cytotoxicity of Jurkat cells is between 10-15 µg/mL in the presence of 2ng/mL of hFasL.

Endotoxin-Niveau: < 0.1 ng per µg (1 EU/µg).

Anwendungsinformationen

Applikationshinweise: Each Investigator should determine their own optimal working dilution for specific applications.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute in water to a concentration of 0.1-1.0 mg/mL.

Buffer: Supplied lyophilized with no additives.

Konservierungsmittel: Without preservative

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: RT/-20 °C

Antigendetails

Target: FAS (TNF Receptor Superfamily, Member 6 (FAS))

Andere Bezeichnung: Fas Receptor

Hintergrund: Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and 145 amino acid cytoplasmic domain.
Alternative Names: sFasR protein, soluble Fas receptor protein, Apo I protein, CD95 protein, Fas Antigen protein, TNFRSF6 protein

Molekulargewicht: 17.6 kDa

Pathways: p53 Signalweg, Apoptose, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity