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FKBP3 Protein (AA 1-224)

Dieses Recombinant FKBP3-Protein wird in Escherichia coli (E. coli) produziert.
Produktnummer ABIN935093

Kurzübersicht für FKBP3 Protein (AA 1-224) (ABIN935093)

Target

Alle FKBP3 Proteine anzeigen
FKBP3 (FK506 Binding Protein 3, 25kDa (FKBP3))

Protein-Typ

Recombinant

Biologische Aktivität

Active

Spezies

  • 8
  • 2
  • 2
  • 1
  • 1
Human

Quelle

  • 9
  • 3
  • 1
  • 1
Escherichia coli (E. coli)

Applikation

SDS-PAGE (SDS)

Reinheit

> 90 % pure
  • Proteineigenschaft

    AA 1-224

    Sequenz

    MAAAVPQRAW TVEQLRSEQL PKKDIIKFLQ EHGSDSFLAE HKLLGNIKNV AKTANKDHLV TAYNHLFETK RFKGTESISK VSEQVKNVKL NEDKPKETKS EETLDEGPPK YTKSVLKKGD KTNFPKKGDV VHCWYTGTLQ DGTVFDTNIQ TSAKKKKNAK PLSFKVGVGK VIRGWDEALL TMSKGEKARL EIEPEWAYGK KGQPDAKIPP NAKLTFEVEL VDID

    Produktmerkmale

    Purified recombinant Human FKBP3 protein
    Expression System: E.coli
    Bioactivity: Specific activity is > 120 nM/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin.
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  • Applikationshinweise

    FKBP3 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

    Testdurchführung

    1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
      2. Add recombinant FKBP3 protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
      3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
      4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
      5. Record the increase in A410 nm for 20 minutes

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Liquid

    Konzentration

    1 mg/mL

    Buffer

    Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT and 10 % glycerol.

    Konservierungsmittel

    Dithiothreitol (DTT)

    Vorsichtsmaßnahmen

    This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

    Handhabung

    Avoid repeated freeze/thaw cycles.

    Lagerung

    RT/-20 °C

    Informationen zur Lagerung

    Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
  • Target

    FKBP3 (FK506 Binding Protein 3, 25kDa (FKBP3))

    Andere Bezeichnung

    FKBP3

    Hintergrund

    FK506 binding protein 3 (FKBP3), also known as FKBP25, is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBP3 associates with transcriptional repressor protein YY1 and histone deaceltylases, HDAC1 and HDAC2. Also, FKBP3 may contain several casein kinase II phosphorylation sites, which are believed to be important for cell growth regulation. It is localized in the nucleus and is expressed in the brain, testis, ovary, and spleen. Recombinant human FKBP3 was expressed in E. coli and purified by using conventional chromatography techniques.
    Alternative Names: PPIase protein, FK506 binding protein 3 FKBP 25 protein, FKBP-3 protein, FKBP3, PPIase FKBP3 protein, FKBP-3, Rotamase protein, Rotamase., FKBP25 protein, FKBP 3, FKBP 3 protein, FKBP 3 protein

    Molekulargewicht

    25.1 kDa (224 AA)
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