Recombinant Human Peptidyl-prolyl cis-trans isomerase FKBP3 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asp224) of Human FKBP3/FKBP25 fused with a 6His tag at the N-terminus..
Reinheit
> 95 % as determined by reducing SDS-PAGE.
Sterilität
0.2 μm filtered
Endotoxin-Niveau
Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
FKBP3
Spezies: Maus
Wirt: Escherichia coli (E. coli)
Recombinant
> 95 %
SDS, WB, ELISA, IP
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Rekonstitution
It is not recommended to reconstitute to a concentration less than 100 μg/mL. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Buffer
Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,1 mM DTT,10 % glycerol .
Konservierungsmittel
Dithiothreitol (DTT)
Vorsichtsmaßnahmen
This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
Lagerung
-80 °C
Informationen zur Lagerung
Store at < -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
FKBP3 contains 1 PPIase FKBP-type domain, belongs to the FKBP-type PPIase family. FK506- and rapamycin-binding proteins (FKBPs) constitute a family of receptors for the two immunosuppressants which inhibit T-cell proliferation by arresting two distinct cytoplasmic signal transmission pathways. FKBP3 is a cis-trans prolyl isomerase enzyme that binds the immunosuppressants FK506 and rapamycin, as well as histone deacetylases, the transcription factor YY1, casein kinase II, and nucleolin. It has a higher affinity for rapamycin than for FK506 and thus may be an important target molecule for immunosuppression by rapamycin. Synonyms: Peptidyl-prolyl cis-trans isomerase FKBP3,PPIase FKBP3,25 kDa FK506-binding protein,25 kDa FKBP,FKBP-25,FK506-binding protein 3,FKBP-3,Immunophilin FKBP25,Rapamycin-selective 25 kDa immunophilin,Rotamase,FKBP25