GSTA1 Protein (AA 1-222)

Produktnummer ABIN935078

Dieses Recombinant GSTA1-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für GSTA1 Protein (AA 1-222) (ABIN935078)

Target: GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 90 % pure

Produktdetails

Proteineigenschaft: AA 1-222

Sequenz: MAEKPKLHYF NARGRMESTR WLLAAAGVEF EEKFIKSAED LDKLRNDGYL MFQQVPMVEI DGMKLVQTRA ILNYIASKYN LYGKDIKERA LIDMYIEGIA DLGEMILLLP VCPPEEKDAK LALIKEKIKN RYFPAFEKVL KSHGQDYLVG NKLSRADIHL VELLYYVEEL DSSLISSFPL LKALKTRISN LPTVKKFLQP GSPRKPPMDE KSLEEARKIF RF

Produktmerkmale: Purified recombinant Human GSTA1 protein
Expression System: E.coli
Bioactivity: Specific activity is 5-8 units/mg, and is defined as the amount of enzyme that conjugate 1.0 uM of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at pH 6.5 at 25 °C.

Anwendungsinformationen

Applikationshinweise: GSTA1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Testdurchführung:

1. Prepare a 1 mL reaction mix into a suitable container: The final concentrations are 97 mM potassium phosphate, 0.97 mM EDTA,
   2.5 mM glutathione, reduced, 1.0 mM 1-chloro-2,4-dinitrobenzene (CDNB),
   3.2 % (v/v) ethanol.
   2. Equilibrate to 25 °C and monitor at A340 nm until the value is constant using a spectrophotometer.
   3. Add 50 µL of recombinant GSTA1 protein with various concentrations (1 µg, 1 µg, 1 µg) in 950 µL reaction buffer.
   4. Mix by inversion and record the increase at A340 nm for 5 minutes.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT and 10 % glycerol.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: RT/-20 °C

Informationen zur Lagerung: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Antigendetails

Target: GSTA1 (Glutathione S-Transferase alpha 1 (GSTA1))

Andere Bezeichnung: GSTA1

Hintergrund: GSTA1, also known as Glutathione S-transferase alpha 1, belongs to a glutathione S-transferase alpha class and is the most abundantly expressed in liver. This protein functions in the detoxification of electrophilic compounds, including carcinogens, therapeutic drugs, environmental toxins and products of oxidative stress, by conjugation with glutathione. Recombinant human GSTA1 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: GSTA-1 protein, GSTA 1 protein, HA subunit 1., GTH1 protein, Glutathione S-transferase alpha 1 Glutathione S transferase A1 protein, GSTA1 1 protein, GSTA1, GSTA-1, GST epsilon protein, GSTA1-1 protein, GST2 protein, GSTA 1, GSTA1 protein

Molekulargewicht: 25.6 kDa (222 AA)