CA2 Protein (AA 1-260)

Produktnummer ABIN935038

Recombinant CA2-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für CA2 Protein (AA 1-260) (ABIN935038)

Target: CA2 (Carbonic Anhydrase II (CA2))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS)

Reinheit: > 95 % pure

Produktdetails

Proteineigenschaft: AA 1-260

Sequenz: MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL KNRQIKASFK

Produktmerkmale: Purified recombinant Human Carbonic anhydrase II protein
Expression System: E.coli
Bioactivity: Specific activity is 50-70 nmoles/min/µg and was obtained by measuring the increase in the amount of p-nitropheno by its esterase activity. Specific activity is defined as the amount of p-nitrophenol that 1ug of enzyme can reduce at 25 °C for 1 minute.
Molecular weight on SDS-PAGE will appear higher.

Anwendungsinformationen

Applikationshinweise: Carbonic anhydrase II protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.

Testdurchführung:

1. Prepare assay buffer into a suitable container: The final concentrations are 12.5 mM Tris, 75 mM NaCl, pH 7.5.
   2. Dilute 4-Nitrophenyl-Acetate(4-NPA) to 2 mM in Assay Buffer.
   3. Dilute recombinant CA2 protein with various concentrations (1 µg, 1 µg, 1 µg) in 50 µL assay buffer.
   4. Load 50 uL of 2 mM 4-NPA into diluted recombinant CA2 solutions, and start the reaction by adding 50 uL of 2 mM Substrate.
   5. Read at a wavelength of 405 nm (bottom read) in kinetic mode for 5 minutes.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM DTT, 50 mM NaCl, and 10 % glycerol.

Konservierungsmittel: Dithiothreitol (DTT)

Vorsichtsmaßnahmen: This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: RT/-20 °C

Informationen zur Lagerung: Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.

Antigendetails

Target: CA2 (Carbonic Anhydrase II (CA2))

Andere Bezeichnung: Carbonic anhydrase II

Hintergrund: Carbonic anhydrase 2 (CA2) is one of fourteen forms of human alpha carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. CA2 is a cytosolic enzyme with the highest activity among all known CAs. Mutations in the CA2 gene result in the CA2 deficiency syndrome, an autosomal recessive disorder that produces osteopetrosis, renal tubular acidosis and cerebral calcification. Recombinant human CA2 was expressed in E. coli and purified by using conventional chromatography.
Alternative Names: Car 2 protein, Carbonic anhydrase II protein, CA II protein, Car2 protein, CAC protein, CA-II protein, CA 2 protein, Carbonic anhydrase 2 protein, Carbonic anhydrase B protein, Carbonate dehydratase II protein, CAI protein, Carbonic anhydrase C protein, Carbonic dehydratase., CA2 protein

Molekulargewicht: 29.2 kDa (260 AA)