YES1 Protein

Produktnummer ABIN7939737

Dieses Recombinant YES1-Protein wird in Baculovirus infected Insect Cells exprimiert.

Kurzübersicht für YES1 Protein (ABIN7939737)

Target: YES1 (V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Applikation: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Produktdetails

Verwendungszweck: Recombinant YES1 protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant YES1 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: YES1 (V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1))

Andere Bezeichnung: YES1

Hintergrund:

Protein Background: YES1 (YES Proto-Oncogene 1, Src Family Tyrosine Kinase), a non-receptor protein tyrosine kinase that is involved in the regulation of cell growth and survival, apoptosis, cell-cell adhesion, cytoskeleton remodeling, and differentiation. Stimulation by receptor tyrosine kinases (RTKs) including EGRF, PDGFR, CSF1R and FGFR leads to recruitment of YES1 to the phosphorylated receptor, and activation and phosphorylation of downstream substrates. Upon EGFR activation, YES1 promotes the phosphorylation of PARD3 to favor epithelial tight junction assembly and participates in the phosphorylation of specific junctional components such as CTNND1 by stimulating the FYN and FER tyrosine kinases at cell-cell contacts. Upon T-cell stimulation by CXCL12, YES1 phosphorylates collapsin response mediator protein 2/DPYSL2 and induces T-cell migration and participates in CD95L/FASLG signaling pathway and mediates AKTmediated cell migration. YES1 plays a role in cell cycle progression by phosphorylating the cyclin-dependent kinase 4/CDK4 thus regulating the G1 phase.

Short Description: Recombinant YES1 protein was expressed in baculovirus expression system as the full length protein (accession number NP_005424.1) with a N-terminal FLAG tag. The molecular weight of the protein is 62.1 kDa.

Aliases: YES Proto-Oncogene 1 , Src Family Tyrosine Kinase

Pathways: CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling