YES1 Protein (His-GST)

Produktnummer ABIN7317062

Recombinant YES1-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für YES1 Protein (His-GST) (ABIN7317062)

Target: YES1 (V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 80 % as determined by reducing SDS-PAGE.

Produktdetails

Aufreinigungstag / Konjugat: Dieses YES1 Protein ist gelabelt mit His-GST.

Verwendungszweck: Recombinant Human c-Yes/YES1 Protein (His & GST Tag)

Sequenz: Gly 2-Leu 543

Produktmerkmale: A DNA sequence encoding the human YES1 (NP_005424.1) (Gly 2-Leu 543) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterilität: 0.2 μm filtered

Endotoxin-Niveau: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 35 nmol/min/mg using Poly(Glu, Tyr) 4:1 as substrate.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, 10 % glycerol, 0.5 mM TCEP, pH 8.0

Lagerung: -20 °C

Informationen zur Lagerung: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Haltbarkeit: 6 months

Antigendetails

Target: YES1 (V-Yes-1 Yamaguchi Sarcoma Viral Oncogene Homolog 1 (YES1))

Andere Bezeichnung: c-Yes/YES1

Hintergrund: HsT441,P61-YES,Yes,c-yes,Proto-oncogene tyrosine-protein kinase Yes, also known as Proto-oncogene c-Yes, p61-Yes and YES1, is a cytoplasm protein which belongs to theprotein kinase superfamily, Tyr protein kinase family and SRC subfamily. YES1 / c-Yes contains oneprotein kinase domain, oneSH2 domain and oneSH3 domain. It is thought that the subcellular distribution of Src-family tyrosine kinases, including c-Yes binding to the cellular membrane, is membranous and/or cytoplasmic. YES1 / c-Yes protein tyrosine kinase is known to be related to malignant transformation. YES1 / c-Yes and c-Src are the two most closely related members of the Src family of nonreceptor tyrosine kinases. Although there is much evidence to support redundancy in signaling between these two kinases. YES1 / c-Yes promotes formation of the tight junction by phosphorylating occludin, while c-Src signaling downregulates occludin formation in a Raf-1 dependent manner. YES1 / c-Yes has tyrosine kinase activity. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP / CD46.

Molekulargewicht:

Calculated MW: 88.5 kDa

Observed MW: 75 kDa

Gen-ID: 7525

NCBI Accession: NP_005424

UniProt: P07947

Pathways: CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling