Ubiquitin Protein (Ub) (AA 1-76)

Produktnummer ABIN7939667

Recombinant Ubiquitin-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für Ubiquitin Protein (Ub) (AA 1-76) (ABIN7939667)

Target: Ubiquitin (Ub)

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Produktdetails

Proteineigenschaft: AA 1-76

Verwendungszweck: Recombinant Ubiquitin (1-76) protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant Ubiquitin (1-76) protein is supplied in 25 mM Tris pH 8.0, 150 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: Ubiquitin (Ub)

Andere Bezeichnung: Ubiquitin

Hintergrund:

Protein Background: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

Short Description: Recombinant Ubiquitin (1-76) protein that includes amino acids 1-76 of human UBC protein (accession number NP_066289.3) was expressed in E. coli and without any tag with a molecular weight of 8.56 kDa. The purity of the protein is ≥ 95 % by SDS-PAGE.

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway