PAK4 Protein

Produktnummer ABIN7939404

Dieses Recombinant PAK4-Protein wird in Baculovirus infected Insect Cells exprimiert.

Kurzübersicht für PAK4 Protein (ABIN7939404)

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Applikation: Functional Studies (Func), Activity Assay (AcA), Screening Assay (ScA)

Produktdetails

Verwendungszweck: Recombinant PAK4 protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant PAK4 protein is supplied in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 10 % glycerol, 0.04 % Triton X-100, 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Andere Bezeichnung: PAK4

Hintergrund:

Protein Background: PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. PAK proteins are critical effectors that link Rho GTPases to cytoskeleton reorganization and nuclear signaling. They serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK4 plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. PAK4 phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. PAK4 phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. PAK4 phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. PAK4 phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers.

Short Description: Recombinant PAK4 protein was expressed in baculovirus expression system as the full length protein (accession number NP_001014831.1) with a N-terminal FLAG tag. The molecular weight of the protein is 65.3 kDa.

Aliases: P21 (RAC1) Activated Kinase 4

Pathways: RTK Signalweg