MBD4 Protein (Methyl-CpG-binding domain)

Produktnummer ABIN7939275

Dieses Recombinant MBD4-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für MBD4 Protein (Methyl-CpG-binding domain) (ABIN7939275)

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Produktdetails

Proteineigenschaft: Methyl-CpG-binding domain

Verwendungszweck: Recombinant MBD4-MBD protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant MBD4-MBD protein is supplied in 25 mM Tris 8.0, 300 mM NaCl, 20 % glycerol, 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: MBD4 (Methyl-CpG Binding Domain Protein 4 (MBD4))

Andere Bezeichnung: MBD4

Hintergrund:

Protein Background: MBD4 (Methyl-CpG-binding domain protein 4) can mismatch-specific DNA N-glycosylase involved in DNA repair. Has thymine glycosylase activity and is specific for G:T mismatches within methylated and unmethylated CpG sites. Can also remove uracil or 5-fluorouracil in G:U mismatches. Has no lyase activity. Was first identified as methylCpG-binding protein.

Short Description: Recombinant MBD4-MBD protein that includes amino acids 71-159 of human MBD4 protein (accession number NP_003916.1) was expressed in E. coli cells with an N-terminal 6xHis tag. The molecular weight of the protein is 12.31 kDa.

Aliases: Methyl-CpG-binding domain protein 4, MED1

Pathways: DNA Reparatur