Kdm6b Protein (AA 1043-1682)

Produktnummer ABIN7939251

Dieses Recombinant Kdm6b-Protein wird in Baculovirus infected Insect Cells exprimiert.

Kurzübersicht für Kdm6b Protein (AA 1043-1682) (ABIN7939251)

Target: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Applikation: Enzyme Kinetics (EK), Screening Assay (ScA)

Produktdetails

Proteineigenschaft: AA 1043-1682

Verwendungszweck: Recombinant JMJD3 / KDM6B (1043-1682) protein

Produktmerkmale: Recombinant JMJD3 / KDM6B (1043-1682) includes amino acids 1043-1682 of human JMJD3 / KMD6B protein (accession number NP_001073893.1), it was expressed in a baculovirus expression system and contains a C-terminal Flag-Tag with a molecular weight of 73.4 kDa. The recombinant protein is ≥ 90 % pure by SDS-PAGE. It is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling.

Anwendungsinformationen

Applikationshinweise: Recombinant JMJD3 / KDM6B (1043-1682) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HDM Activity Assay Conditions: 1 uM H3K27me3 peptide was incubated with different concentrations of JMJD3 (1043- 1682) protein in 10 µL reaction system containing 50 mM HEPES-NaOH pH 7.4, 0.02 % Triton X-100, 1 mM TCEP, 50 uM 2-OG, 50 uM Ascorbate and 25 uM (NH4)2Fe(SO4)2•6H2O for 2 hours, then 10 micro,l H3K27me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: Recombinant JMJD3 / KDM6B (1043-1682) protein is supplied at a concentration of 0.4 μg/ul in 25 mM HEPES-NaOH pH 7.5, 300 mM NaCl, 5 % glycerol, 0.04 % Triton X-100, 0.2 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: Kdm6b (Lysine (K)-Specific Demethylase 6B (Kdm6b))

Andere Bezeichnung: JMJD3 & KDM6B

Hintergrund:

Protein Background: JMJD3 / KDM6B (Jumonji domain-containing protein 3 lysine (K)-specific demethylase 6B) is a histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby, playing a central role in histone code. JMJD3 demethylates trimethylated and dimethylated H3 'Lys-27'. It plays a central role in regulation of posterior development by regulating HOX gene expression. JMJD3 / KDM6B is involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation.

Short Description: Recombinant JMJD3 / KDM6B (1043-1682) that includes amino acids 1043 - 1682 of human JMJD3 / KDM6B protein (accession number NP_001073893.1) was expressed in Baculovirus expression system and contains a C-terminal Flag tag with a molecular weight of 73.4 kDa. The recombinant protein is ≥ 90 % pure by SDS-PAGE.

Aliases: Jumonji Domain Containing 3 Histone Lysine Demethylase, KDM6B, Lysine Demethylase 6B

Molekulargewicht: 73.4 kDa

NCBI Accession: NP_001073893

Pathways: Warburg Effekt