CRISPR-Cas9 (full length) protein (His tag)

Produktnummer ABIN7938731

Dieses Recombinant CRISPR-Cas9-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für CRISPR-Cas9 (full length) protein (His tag) (ABIN7938731)

Target: CRISPR-Cas9

Protein-Typ: Recombinant

Spezies: Streptococcus pyogenes

Quelle: Escherichia coli (E. coli)

Applikation: Activity Assay (AcA), Functional Studies (Func), Screening Assay (ScA)

Produktdetails

Proteineigenschaft: full length

Aufreinigungstag / Konjugat: His tag

Verwendungszweck: Recombinant Cas9 protein

Produktmerkmale: Recombinant Cas9 protein was expressed in E. coli as the full-length protein (accession number WP_010922251.1) with an N-terminal 6xHis Tag and a C-terminal nuclear localization sequence. The molecular weight of the protein is 161.2 kDa. This protein is useful for the study of in vitro and in vivo DNA endonuclease reactions as well as other genome engineering.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Recombinant Cas9 protein is supplied in 25 mM Tris pH 8.0, 300 mM NaCl, 20 % glycerol and 0.5 mM TCEP.

Handhabung: Avoid repeated freeze/thaw cycles,keep on ice when not in storage

Lagerung: -80 °C

Informationen zur Lagerung: Recombinant proteins in solution are temperature sensitive and must be stored at -80°C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.

Antigendetails

Target: CRISPR-Cas9

Andere Bezeichnung: Cas9

Hintergrund:

Protein Background: Type II CRISPR (Clustered Regularly Interspersed Short Palindromic Repeats) RNA-guided endonuclease Cas9 (CRISPR associated protein 9) is an RNA-guided DNA endonuclease associated with adaptive immunity system in Streptococcus pyogenes, among other bacteria. S. pyogenes utilizes Cas9 to memorize and later interrogate and cleave foreign DNA, such as invading bacteriophage DNA or plasmid DNA. Cas9 performs this interrogation by unwinding foreign DNA and checking for sites complementary to the 20 basepair spacer region of the guide RNA. If the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA. In this sense, the CRISPR-Cas9 mechanism has a number of parallels with the RNA interference (RNAi) mechanism in eukaryotes. Apart from its original function in bacterial immunity, the Cas9 protein has been heavily utilized as a genome engineering tool to induce site-directed double strand breaks in DNA. These breaks can lead to gene inactivation or the introduction of heterologous genes through non-homologous end joining and homologous recombination respectively in many laboratory model organisms.

Short Description: Recombinant Cas9 protein was expressed in E.coli as the full length protein (accession number WP_010922251.1) with an N-terminal 6x His tag and a C-terminal nuclear localization sequence. The molecular weight of the protein is 161.2 kDa.

Aliases: Clustered Regularly Interspersed Short Palindromic Repeasts, CRISPR associated protein 9

Molekulargewicht: 161.2 kDa

NCBI Accession: WP_010922251