CRISPR-Cas9 protein (His tag)

Produktnummer ABIN7884339

Recombinant CRISPR-Cas9-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für CRISPR-Cas9 protein (His tag) (ABIN7884339)

Target: CRISPR-Cas9

Protein-Typ: Recombinant

Spezies: Streptococcus pyogenes

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB)

Reinheit: >98 %

Produktdetails

Aufreinigungstag / Konjugat: His tag

Verwendungszweck: wtCas9

Sequenz: MDKKYSIGLD IGTNSVGWAV ITDEYKVPSK KFKVLGNTDR HSIKKNLIGA LLFDSGETAE ATRLKRTARR RYTRRKNRIC YLQEIFSNEM AKVD DSFFHRLEES FLVEEDKKHE RHPIFGNIVD EVAYHEKYPT IYHLRKKLVD STDKADLRLI YLALAHMIKF RGHFLIEGDL NPDNSDVDKL FIQLVQ TYNQLFEENP INASGVDAKA ILSARLSKSR RLENLIAQLP GEKKNGLFGN LIALSLGLTP NFKSNFDLAE DAKLQLSKDT YDDDLDNLLA QIGD QYADLFLAAK NLSDAILLSD ILRVNTEITK APLSASMIKR YDEHHQDLTL LKALVRQQLP EKYKEIFFDQ SKNGYAGYID GGASQEEFYK FIKPILE KMDGTEELLV KLNREDLLRK QRTFDNGSIP HQIHLGELHA ILRRQEDFYP FLKDNREKIE KILTFRIPYY VGPLARGNSR FAWMTRKSEE TITPW NFEEVVDKGA SAQSFIERMT NFDKNLPNEK VLPKHSLLYE YFTVYNELTK VKYVTEGMRK PAFLSGEQKK AIVDLLFKTN RKVTVKQLKE DYFK KIECFDSVEI SGVEDRFNAS LGTYHDLLKI IKDKDFLDNE ENEDILEDIV LTLTLFEDRE MIEERLKTYA HLFDDKVMKQ LKRRRYTGWG RLSRKLI NGIRDKQSGK TILDFLKSDG FANRNFMQLI HDDSLTFKED IQKAQVSGQG DSLHEHIANL AGSPAIKKGI LQTVKVVDEL VKVMGRHKPE NIV IEMARENQTT QKGQKNSRER MKRIEEGIKE LGSQILKEHP VENTQLQNEK LYLYYLQNGR DMYVDQELDI NRLSDYDVDH IVPQSFLKDD SI DNKVLTRSDK NRGKSDNVPS EEVVKKMKNY WRQLLNAKLI TQRKFDNLTK AERGGLSELD KAGFIKRQLV ETRQITKHVA QILDSRMNTK Y DENDKLIREV KVITLKSKLV SDFRKDFQFY KVREINNYHH AHDAYLNAVV GTALIKKYPK LESEFVYGDY KVYDVRKMIA KSEQEIGKAT AKYFF YSNIMNFFKT EITLANGEIR KRPLIETNGE TGEIVWDKGR DFATVRKVLS MPQVNIVKKT EVQTGGFSKE SILPKRNSDK LIARKKDWDP KKYG GFDSPTVAYS VLVVAKVEKG KSKKLKSVKE LLGITIMERS SFEKNPIDFL EAKGYKEVKK DLIIKLPKYS LFELENGRKR MLASAGELQK GNELALP SKYVNFLYLA SHYEKLKGSP EDNEQKQLFV EQHKHYLDEI IEQISEFSKR VILADANLDK VLSAYNKHRD KPIREQAENI IHLFTLTNLG APAAFK YFDTTIDRKR YTSTKEVLDA TLIHQSITGL YETRIDLSQL GGD with polyhistid ine tag at the C-terminus

Endotoxin-Niveau: <0.1 EU per 1 μg protein

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: dissolve in sterile H2O

Buffer: PBS, pH 7.4

Lagerung: -20 °C

Informationen zur Lagerung: -20°C

Antigendetails

Target: CRISPR-Cas9

Andere Bezeichnung: Cas9

Hintergrund: The wtCas9 is a recombinant Streptococcus pyogenes Cas9 protein (wild type form) purified from E. coli. Cas9 protein can form a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA). The RNP complex recognizes the target site by matching gRNA with the genomic DNA sequence and leads to DNA breaks within 3 bases from the NGG PAM (Protospacer Adjacent Motif). With the CRISPR/Cas9 system, researchers can valid gene editing assay in cells or other kinds of animals.

Molekulargewicht: 158kDa

UniProt: Q99ZW2