Sonic Hedgehog Protein (SHH) (N-Term)
Kurzübersicht für Sonic Hedgehog Protein (SHH) (N-Term) (ABIN7937273)
Target
Alle Sonic Hedgehog (SHH) Proteine anzeigenProtein-Typ
Biologische Aktivität
Spezies
Quelle
Reinheit
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Proteineigenschaft
- N-Term
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Verwendungszweck
- Recombinant Human Sonic Hedgehog N-Terminus
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Sequenz
- IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG
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Sterilität
- 0.2 μm filtered
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Endotoxin-Niveau
- Less than 1 EU/ug of rHuSHH as determined by LAL method.
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Biological Activity Comment
- Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H/10T1/2 cells is less than 1 μg/ml, corresponding to a specific activity of > 1.0 x103 IU/mg.
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Sonic Hedgehog (SHH) (AA 24-197), (Cys24IleIle-Mutant) (Active) protein
SHH Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 25-198), (Cys25IleIle-Mutant) (Active) proteinSHH Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Sonic Hedgehog (SHH) (AA 181-280) protein (GST tag)SHH Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AP, AA
Sonic Hedgehog (SHH) protein (His tag)SHH Spezies: Maus Wirt: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Sonic Hedgehog (SHH) (Active) protein (His tag)SHH Spezies: Maus Wirt: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) protein (His tag)SHH Spezies: Human Wirt: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE. AM, ADA, IA, ScA
Sonic Hedgehog (SHH) (AA 25-198) protein (His tag)SHH Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 95 % SDS
Sonic Hedgehog (SHH) (Active) proteinSHH Spezies: Maus Wirt: Escherichia coli (E. coli) Recombinant > 95 % as determined by SDS-PAGE Active
Sonic Hedgehog (SHH) (AA 24-197) protein (His tag)SHH Spezies: Human Wirt: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE.
Sonic Hedgehog (SHH) proteinSHH Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant 97,00% SDS
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Kommentare
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Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Buffer
- Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
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Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.- 12 months from date of receipt, -20 to -70 °C as supplied.- 1 month, 2 to 8 °C under sterile conditions after reconstitution.- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Haltbarkeit
- 12 months
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- Sonic Hedgehog (SHH)
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Andere Bezeichnung
- Sonic Hedgehog
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Molekulargewicht
- 19.8 kDa
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Gen-ID
- 6469
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UniProt
- Q15465
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Pathways
- Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
Target
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