WISP1 Protein (AA 23-367)

Produktnummer ABIN7866102

Dieses Recombinant WISP1-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für WISP1 Protein (AA 23-367) (ABIN7866102)

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: >95 % , as determined by SDS-PAGE and HPLC

Produktdetails

Proteineigenschaft: AA 23-367

Verwendungszweck: Human WISP1 Protein Recombinant

Sequenz: TALSPAPTTM DFTPAPLEDT SSRPQFCKWP CECPPSPPRC PLGVSLITDG CECCKMCAQQ LGDNCTEAAI CDPHRGLYCD YSGDRPRYAI GVCAQVVGVG CVLDGVRYNN GQSFQPNCKY NCTCIDGAVG CTPLCLRVRP PRLWCPHPRR VSIPGHCCEQ WVCEDDAKRP RKTAPRDTGA FDAVGEVEAW HRNCIAYTSP WSPCSTSCGL GVSTRISNVN AQCWPEQESR LCNLRPCDVD IHTLIKAGKK CLAVYQPEAS MNFTLAGCIS TRSYQPKYCG VCMDNRCCIP YKSKTIDVSF QCPDGLGFSR QVLWINACFC NLSCRNPNDI FADLESYPDF SEIAN

Produktmerkmale:

Protein Size: Recombinant WISP1 migrates as an approximately 38 kDa protein under reducing conditions in SDS-PAGE.

Sequence Position: Optimized DNA sequence encoding Human WISP1 mature chain including a C-terminal 6xHis tag was expressed in E. Coli. Recombinant Human WNT1-inducible-signaling pathway protein 1 encodes amino acid residues 23-367.

Endotoxin-Niveau: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Biological Activity Comment: Determined by the dose-dependant proliferation of the MCF-7 cell line. The expected ED50 for this effect is 1.0-3.0 μg/ml.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: WISP1 was Lyophilized from PBS, pH 7.2.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 24 months

Antigendetails

Target: WISP1 (WNT1 Inducible Signaling Pathway Protein 1 (WISP1))

Andere Bezeichnung: WISP1

Molekulargewicht: 38 kDa

Pathways: WNT Signalweg, Growth Factor Binding