Sonic Hedgehog Protein (SHH) (AA 24-462)

Produktnummer ABIN7866065

Recombinant Sonic Hedgehog-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für Sonic Hedgehog Protein (SHH) (AA 24-462) (ABIN7866065)

Target: Sonic Hedgehog (SHH)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: >95 % , as determined by SDS-PAGE and HPLC

Produktdetails

Proteineigenschaft: AA 24-462

Verwendungszweck: Human SHH Protein Recombinant

Sequenz: IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKISRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRALDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG

Produktmerkmale:

Protein Size: Recombinant SHH migrates as an approximately 19 kDa protein under reducing conditions in SDS-PAGE.

Sequence Position: Optimized DNA sequence encoding Human SHH mature chain was expressed in E. Coli. Recombinant Human Sonic hedgehog protein encodes amino acid residues 24-462.

Endotoxin-Niveau: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/μg(1EU/μg).

Biological Activity Comment: Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.8-1.0 μg/ml.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

Buffer: SHH was Lyophilized from PBS, pH 7.2.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The lyophilized protein is stable for at least 2 years from date of receipt at -20°C. Upon reconstitution, this product can be stored in working aliquots at 2°- 8°C for one month, or at -20°C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 24 months

Antigendetails

Target: Sonic Hedgehog (SHH)

Andere Bezeichnung: SHH

Molekulargewicht: 19 kDa

Pathways: Hedgehog Signalweg, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development