Ubiquitin Protein (Ub) (His tag)
Kurzübersicht für Ubiquitin Protein (Ub) (His tag) (ABIN7669930)
Target
Alle Ubiquitin (Ub) Proteine anzeigenProtein-Typ
Biologische Aktivität
Spezies
Quelle
Reinheit
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Aufreinigungstag / Konjugat
- Dieses Ubiquitin Protein ist gelabelt mit His tag.
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Verwendungszweck
- Recombinant Human Ub protein (His tag)
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Sequenz
- Lys 103-Cys 229
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Aufreinigung
- > 95 % as determined by reducing SDS-PAGE.
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Sterilität
- 0.2 μm filtered
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Endotoxin-Niveau
- < 10 EU/mg of the protein as determined by the LAL method.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
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Buffer
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Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
Lagerung
- 4 °C,-20 °C,-80 °C
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Informationen zur Lagerung
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Haltbarkeit
- 12 months
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- Ubiquitin (Ub)
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Andere Bezeichnung
- Ub
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Hintergrund
- Polyubiquitin-B(UBB) is one of Ubiquitins. Ubiquitin is one of the most conserved proteins known in eukaryotic organisms. Ubiquitin is required for ATP-dependent, non-lysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation.,Polyubiquitin-B,UBB,Ubiquitin,Ubiquitin B
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Molekulargewicht
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calculated_mw: 13.9 kDa
observed_mw: 15 kDa
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UniProt
- P0CG47
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Pathways
- Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
Target
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