IL-21 Protein (AA 18-146)

Produktnummer ABIN7596303

Recombinant IL-21-Protein exprimiert in Escherichia coli (E. coli).

Kurzübersicht für IL-21 Protein (AA 18-146) (ABIN7596303)

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: Escherichia coli (E. coli)

Applikation: SDS-PAGE (SDS), Activity Assay (AcA)

Reinheit: > 90% by SDS - PAGE

Produktdetails

Proteineigenschaft: AA 18-146

Sequenz: MHKSSPQGPD RLLIRLRHLI DIVEQLKIYE NDLDPELLSA PQDVKGHCEH AAFACFQKAK LKPSNPGNNK TFIIDLVAQL RRRLPARRGG KKQKHIAKCP SCDSYEKRTP KEFLERLKWL LQKMIHQHLS

Endotoxin-Niveau: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤ 100 ng/ml.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Lagerung: 4 °C,-20 °C,-80 °C

Informationen zur Lagerung: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Antigendetails

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Andere Bezeichnung: IL-21 Protein

Hintergrund: IL-21, also known as Interleukin-21, is produced from activated helper T cells such as Th17 cells and is a member of the common-γ chain family of cytokines that includes IL-2, IL-4, IL-7, IL-9, and IL-15. Previous studies have shown that IL-21 plays an important role in the regulation of both innate and adaptive immune responses. IL-21 is critical for NK cell activation and expansion. IL-21 also promotes terminal B cell differentiation into plasma cells and antibody production. Furthermore, IL-21 enhances antigen-specific CD8 T cell expansion and the differentiation of follicular helper T cells in vivo. IL-21 also promotes the differentiation of the inflammatory Th17 lineage and contributes to autoimmunity. IL-21 isoform is an alternative splice variant form of IL-21 that we previously identified and reported to have a similar function as original IL-21. Follicular helper T cells also produce IL-21 to activate and differentiate B cells. Recombinant mouse IL-21, was expressed in E. coli and purified by using conventional chromatography techniques.

Molekulargewicht: 15 kDa (130aa), confirmed by MALDI-TOF

NCBI Accession: NP_068554

Pathways: JAK-STAT Signalweg, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response