NGF 2.5S (Active) protein (Atto 647N)

Produktnummer ABIN7582347

Dieses Native NGF 2.5S-Protein wird in Maus exprimiert.

Kurzübersicht für NGF 2.5S (Active) protein (Atto 647N) (ABIN7582347)

Target: NGF 2.5S

Protein-Typ: Native

Biologische Aktivität: Active

Spezies: Maus

Quelle: Maus

Applikation: Cell Culture (CC), Cytotoxicity Test (CyTox), Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI), Binding Studies (Bind), Flow Cytometry (FACS)

Produktdetails

Aufreinigungstag / Konjugat: Atto 647N

Verwendungszweck: mouse NGF 2.5S-ATTO Fluor-647N

Spezifität: Toxin target: p75 neurotrophin receptor (p75NTR), TrkA receptors

Produktmerkmale: ATTO 647N (Ex/Em: 646/664 nm). The extent of labeling is one molecule of dye per one mouse NGF 2.5S molecule, linked via primary amines.

Biological Activity Comment: The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75NTR, which binds all the neurotrophins.

Anwendungsinformationen

Applikationshinweise: Antigen Preadsorption Control: 4-100 nM

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Note: The product is typically supplied in a 0.5 mL conical vial. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles and exposure to light to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Handhabung: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid vigorous vortexing. protect from moisture,keep the vial protected from light

Lagerung: -20 °C

Informationen zur Lagerung: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C, protect from moisture, and keep the vial protected from light.

Antigendetails

Target: NGF 2.5S

Hintergrund: A fluorescent mouse NGF 2.5S for sensitive detection, live-cell imaging, and tracking of TrkA and p75NTR receptors and NGF internalization dynamics

Molekulargewicht: ~26.6 kDa (dimer)