NGF 2.5S (Active) protein (Biotin)

Produktnummer ABIN7482121

Native NGF 2.5S-Protein exprimiert in Maus.

Kurzübersicht für NGF 2.5S (Active) protein (Biotin) (ABIN7482121)

Target: NGF 2.5S

Protein-Typ: Native

Biologische Aktivität: Active

Spezies: Maus

Quelle: Maus

Applikation: Cell Culture (CC), Cytotoxicity Test (CyTox), Fluorescence Microscopy (FM), Immunofluorescence (IF), Live Cell Imaging (LCI)

Produktdetails

Aufreinigungstag / Konjugat: Biotin

Verwendungszweck: mouse NGF 2.5S-Biotin

Spezifität: Toxin target: p75 neurotrophin receptor (p75NTR), TrkA receptors

Produktmerkmale: LC-Biotin. The extent of labeling is 1-3 Molecules of biotin per one molecule of mouse NGF 2.5S (Grade I), linked via primary amines.

Biological Activity Comment: The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75NTR, which binds all the neurotrophins.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Handhabung: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Lagerung: -20 °C

Informationen zur Lagerung: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Antigendetails

Target: NGF 2.5S

Hintergrund: A biotinylated mouse NGF 2.5S for imaging and sensitive detection of TrkA and p75NTR receptors

Molekulargewicht: ~26 kDa