DDIT4 Protein (AA 1-229) (His tag)

Produktnummer ABIN7564980

Produktdetails

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-229

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses DDIT4 Protein ist gelabelt mit His tag.

Verwendungszweck: Custom-made recombinant Ddit4 Protein expressed in mammalian cells.

Sequenz: MPSLWDRFSS SSSSSSSSRT PAADRPPRSA WGSAAREEGL DRCASLESSD CESLDSSNSG FGPEEDSSYL DGVSLPDFEL LSDPEDEHLC ANLMQLLQES LSQARLGSRR PARLLMPSQL VSQVGKELLR LAYSEPCGLR GALLDVCVEQ GKSCHSVAQL ALDPSLVPTF QLTLVLRLDS RLWPKIQGLL SSANSSLVPG YSQSLTLSTG FRVIKKKLYS SEQLLIEEC Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Spezifität: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Produktmerkmale: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Reinheit: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Güteklasse: custom-made

Anwendungsinformationen

Applikationshinweise: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: DDIT4 (DNA-Damage-Inducible Transcript 4 (DDIT4))

Andere Bezeichnung: Ddit4 (DDIT4 Produkte)

Hintergrund: DNA damage-inducible transcript 4 protein (Dexamethasone-induced gene 2 protein) (HIF-1 responsive protein RTP801) (Protein regulated in development and DNA damage response 1) (REDD-1),FUNCTION: Regulates cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). Inhibition of mTORC1 is mediated by a pathway that involves DDIT4/REDD1, AKT1, the TSC1-TSC2 complex and the GTPase RHEB. Plays an important role in responses to cellular energy levels and cellular stress, including responses to hypoxia and DNA damage. Regulates p53/TP53-mediated apoptosis in response to DNA damage via its effect on mTORC1 activity. Its role in the response to hypoxia depends on the cell type, it mediates mTORC1 inhibition in fibroblasts and thymocytes, but not in hepatocytes. Inhibits neuronal differentiation and neurite outgrowth mediated by NGF via its effect on mTORC1 activity. Required for normal neuron migration during embryonic brain development. Plays a role in neuronal cell death. Required for mTORC1-mediated defense against viral protein synthesis and virus replication. {ECO:0000269|PubMed:15545625, ECO:0000269|PubMed:15988001, ECO:0000269|PubMed:20176937, ECO:0000269|PubMed:21383064, ECO:0000269|PubMed:21896779, ECO:0000269|PubMed:21909097}.

Molekulargewicht: 24.9 kDa

UniProt: Q9D3F7

AlphaFold: Q9D3F7

Pathways: Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process