Shootin-1 (SHTN1) (AA 1-631) protein (His tag)

Produktnummer ABIN7564637

Produktdetails

Target: Shootin-1 (SHTN1)

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-631

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: His tag

Verwendungszweck: Predefined custom protein recombinant Shtn1 Protein expressed in mammalian cells.

Sequenz: MNSSDEEKQL QLITSLKEQA IGEYEDLRAE NQKTKEKCDK IRQERDEAVK KLEEFQKISH MVIEEVNFMQ NHLEIEKTCR ESAEALATKL NKENKTLKRI SMLYMAKLGP DVITEEINID DDDPATDTDA AAETCVSVQC QKQIKELRDQ IVSVQEEKKV LAIELENLKS KLGEVMEEVN KVKQEKAVLN SEVLEQRKVL EKCNRVSMLA VEEYEELQVN LELEKDLRKK AESFAQEMFI EQNKLKRQSH LLLQSSLPDQ QLLKALDENA KLIQQLEEER IQHQKKVKEL EERLENEALH KEIHNLRQQL ELLEDDKREL EQKYQSSEEK ARNLKHSVDE LQKRVNQSEN SVPPPPPPPP PLPPPPPNPI RSLMSMIRKR SHPSGNSAKK EKTTQPETAE EVTDLKRQAV EEMMDRIKKG VHLRPVNQTA RPKAKPDSLK GSESAVDELK GILGTLNKST SSRSLKSLGP ENSETELERI LRRRKLTAEA DSSSPTGILA TSESKSMPVL GSVSSVTKSA LNKKTLEAEF NNPCPLTPEP GEGPRKLEGC TNPKVTFQPP SKGGYRRKCV GSENQAEPVV VLDPVSTHEP QTKDQAAEKD PTQFEEEGGE TQPEYKEDSG GKTGETDSSN C Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Spezifität: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Produktmerkmale: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Reinheit: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Güteklasse: custom-made

Anwendungsinformationen

Applikationshinweise: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: Shootin-1 (SHTN1)

Andere Bezeichnung: Shtn1 (SHTN1 Produkte)

Hintergrund: Shootin-1 (Shootin1),FUNCTION: Involved in the generation of internal asymmetric signals required for neuronal polarization and neurite outgrowth (PubMed:23864681). Mediates netrin-1-induced F-actin-substrate coupling or 'clutch engagement' within the axon growth cone through activation of CDC42, RAC1 and PAK1-dependent signaling pathway, thereby converting the F-actin retrograde flow into traction forces, concomitantly with filopodium extension and axon outgrowth. Plays a role in cytoskeletal organization by regulating the subcellular localization of phosphoinositide 3-kinase (PI3K) activity at the axonal growth cone. Also plays a role in regenerative neurite outgrowth (By similarity). In the developing cortex, cooperates with KIF20B to promote both the transition from the multipolar to the bipolar stage and the radial migration of cortical neurons from the ventricular zone toward the superficial layer of the neocortex (PubMed:23864681). Involved in the accumulation of phosphatidylinositol 3,4,5-trisphosphate (PIP3) in the growth cone of primary hippocampal neurons (PubMed:23864681). {ECO:0000250|UniProtKB:A0MZ67, ECO:0000269|PubMed:23864681}.

Molekulargewicht: 71.3 kDa

UniProt: Q8K2Q9

AlphaFold: Q8K2Q9