BARD1 Protein (AA 1-765) (His tag)

Produktnummer ABIN7562548

Produktdetails

Target: BARD1 (BRCA1 Associated RING Domain 1 (BARD1))

Protein-Typ: Recombinant

Proteineigenschaft: AA 1-765

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses BARD1 Protein ist gelabelt mit His tag.

Verwendungszweck: Made-to-order recombinant Bard1 Protein expressed in mammalian cells.

Sequenz: MPRRPPRVCS GNQPAPVPAM EPATDGLWAH SRAALARLEK LLRCSRCANI LKEPVCLGGC EHIFCSGCIS DCVGSGCPVC YTPAWILDLK INRQLDSMIQ LSSKLQNLLH DNKDSKDNTS RASLFGDAER KKNSIKMWFS PRSKKVRYVV TKVSVQTQPQ KAKDDKAQEA SMYEFVSATP PVAVPKSAKT ASRTSAKKHP KKSVAKINRE ENLRPETKDS RFDSKEELKE EKVVSCSQIP VMERPRVNGE IDLLASGSVV EPECSGSLTE VSLPLAEHIV SPDTVSKNEE TPEKKVCVKD LRSGGSNGNR KGCHRPTTST SDSCGSNIPS TSRGIGEPAL LAENVVLVDC SSLPSGQLQV DVTLRRKSNA SDDPLSLSPG TPPPLLNNST HRQMMSSPST VKLSSGMPAR KRNHRGETLL HIASIKGDIP SVEYLLQNGN DPNVKDHAGW TPLHEACSHG HLKVVELLLQ HNALVNTPGY QNDSPLHDAV KSGHIDIVKV LLSHGASRNA VNIFGVRPVD YTDNENIRSL LLLPEENESF STSQCSIVNT GQRKNGPLVF IGSGLSSQQQ KMLSKLETVL KAKKCMEFDS TVTHVIVPDE EAQSTLKCML GILSGCWILK FDWVKACLDS KVREQEEKYE VPGGPQRSRL NREQLLPKLF DGCYFFLGGN FKHHPRDDLL KLIAAAGGKV LSRKPKPDSD VTQTINTVAY HAKPESDQRF CTQYIVYEDL FNCHPERVRQ GKVWMAPSTW LISCIMAFEL LPLDS Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Spezifität: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Produktmerkmale: Key Benefits:

• Made to order protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Reinheit: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Güteklasse: custom-made

Anwendungsinformationen

Applikationshinweise: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -80 °C

Informationen zur Lagerung: Store at -80°C.

Haltbarkeit: 12 months

Antigendetails

Target: BARD1 (BRCA1 Associated RING Domain 1 (BARD1))

Andere Bezeichnung: Bard1 (BARD1 Produkte)

Hintergrund: BRCA1-associated RING domain protein 1 (BARD-1) (EC 2.3.2.27) (RING-type E3 ubiquitin transferase BARD1),FUNCTION: E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage. {ECO:0000250|UniProtKB:Q99728}.

Molekulargewicht: 84.3 kDa

UniProt: O70445

AlphaFold: O70445

Pathways: DNA Reparatur