custom-made
HERC6
Spezies: Maus
Wirt: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
HERC6
(HECT and RLD Domain Containing E3 Ubiquitin Protein Ligase Family Member 6 (HERC6))
Protein-Typ
Recombinant
Proteineigenschaft
AA 1-1003
Spezies
Maus
Quelle
HEK-293 Cells
Aufreinigungstag / Konjugat
Dieses HERC6 Protein ist gelabelt mit His tag.
Verwendungszweck
Made-to-order recombinant Herc6 Protein expressed in mammalian cells.
Sequenz
MYFSWAAGSR KPRRLKAGTS GIELLQAASG EHHSLLLFSN HRVYSCGDNS WGQLGQRRDQ STERPEPIQA LNDLHVDLVS CGKEHSVAVC HKGKVFAWGA GSEGQLGIGE FKEISFMPTK IKALAGIKII QVSCGHYHSL ALSEDGHVFS WGRNSEGQLG LGKNSRSQAI PQKVKSLEGI PLAQVAAGGT HSFALSLTGT SFGWGSNRSG QLALSGNKVK EQIYKPHSIG ALKNLSVIYI SCGYEHTAVL TEEGQVFTFG GNSSGQLQPS PRSGQRGPQL IEGIGGRVSQ IECASYHTIA YVYTTGQVVS LGRGPSHTSN PTHQEAPAEN SDITCLLAAE DLVDIEVKDI FAGAHANFVT TRRVSHTRST GVSMKILPEI IRINQSLVKK WRAANKRKDR EGAKREISLI FSSSACLTAS FLKKRDAGEN NLIDVDLKLA RDVFKKLTTE KWISSLITTC LEEYLLRDLP YSSPHQEALL VFLLLPECSI MQDPKNWKTL AFEFAKAIHK MGPQSLAFLR TCWASLEVSS LNILVQMLKK AIISQIQYGV ATEQYITNIK VLLEVIKEVH KANCQLPESA FIINELSGIF NFDAEAGRMF IRHNDLDCTE SSDMVVFSDF LFVFDLPSKI KLMKCDSFVK LMSEVMAFPE KMSSPPYLIL KVRRSHLVED TLRQLRQVED FDLRKQLSVG FINEIRPEAG GVSSEFFHCI FEEMTDPKYE MFIYPEKGSS MWFPVNPKFE KSSYFLFGIL CGLSLHNLKV INLPFPLALY KKLLNQKPSL EDLKELSLPL GRNLQEVLNC EAGDIEELHM YFSIYWDQKD VDLIPDGISV PVNETNKRDY VSKYVDYIFN ISIKTIYEEF HRGFYKVCNW DIIRQFQPEE LMTAIIGNAT CDWKQFENNS KYKDGYDKSH PTILLFWKAF HDLTLDEKKK FLLFLTGCDR LHVKGLQNEG IVFRCSETFS EEDNPRSLTC HRMLDLPKYS SMRRMKEALQ VAINNSTGFV SQE Sequence without tag. The proposed Purification-Tag is based on experiences with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
Spezifität
If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.
Produktmerkmale
Key Benefits:
Made to order protein - from design to production - by highly experienced protein experts.
Protein expressed in mammalian cells and purified in one-step affinity chromatography
The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
If you are not interested in a full length protein, please contact us for individual protein fragments.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Reinheit
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
HERC6
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
custom-made
HERC6
Spezies: Human
Wirt: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, SDS, WB
custom-made
HERC6
Spezies: Maus
Wirt: Cell-free protein synthesis (CFPS)
Recombinant
> 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).
ELISA, SDS, WB
HERC6
Spezies: Human
Wirt: HEK-293 Cells
Recombinant
> 80 % as determined by SDS-PAGE and Coomassie blue staining
AbP, STD
Applikationshinweise
We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
The buffer composition is at the discretion of the manufacturer.
Handhabung
Avoid repeated freeze-thaw cycles.
Lagerung
-80 °C
Informationen zur Lagerung
Store at -80°C.
Haltbarkeit
12 months
Target
HERC6
(HECT and RLD Domain Containing E3 Ubiquitin Protein Ligase Family Member 6 (HERC6))
E3 ISG15--protein ligase Herc6 (EC 2.3.2.-),FUNCTION: Major E3 ligase for ISG15 conjugation. Acts as a positive regulator of innate antiviral response in cells induced by interferon. {ECO:0000269|PubMed:22272257}.