EPH Receptor A2 Protein (EPHA2)

Produktnummer ABIN7533760

Recombinant EPH Receptor A2-Protein exprimiert in Baculovirus infected Insect Cells.

Kurzübersicht für EPH Receptor A2 Protein (EPHA2) (ABIN7533760)

Target: EPH Receptor A2 (EPHA2)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Baculovirus infected Insect Cells

Reinheit: > 95 % by SDS-PAGE.

Produktdetails

Verwendungszweck: Active Recombinant Human EphA2/ECK Protein

Sequenz: DPNQAVLKFT TEIHPSCVTR QKVIGAGEFG EVYKGMLKTS SGKKEVPVAI KTLKAGYTEK QRVDFLGEAG IMGQFSHHNI IRLEGVISKY KPMMIITEYM ENGALDKFLR EKDGEFSVLQ LVGMLRGIAA GMKYLANMNY VHRDLAARNI LVNSNLVCKV SDFGLSRVLE DDPEATYTTS GGKIPIRWTA PEAISYRKFT SASDVWSFGI VMWEVMTYGE RPYWELSNHE VMKAINDGFR LPTPMDCPSA IYQLMMQCWQ QERARRPKFA DIVSILDKLI RAPDSLKTLA DFDPRVSIRL PSTSGSEGVP FRTVSEWLES IKMQQYTEHF MAAGYTAIEK VVQMTNDDIK RIGVRLPGHQ KRIAYSLLGL KDQVNTVGIP I

Spezifität: Asp596-Ile976

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: < 1.0 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Human EphA2 at 1 μg/mL (100 μL/well) can bind Human EFNA1 with a linear range of 4.883 ng/mL-9.206 μg/mL.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Antigendetails

Target: EPH Receptor A2 (EPHA2)

Andere Bezeichnung: EphA2/ECK

Hintergrund:

Description: Eph receptor A2 (Ephrin type-A receptor 2 or EphA2) is a member of the ephrin receptor subfamily of the protein-tyrosine kinase family.The receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration.

Name: ARCC2,CTPA,CTPP1,CTRCT6,ECK,EPHA2

Gen-ID: 1969

UniProt: P29317

Pathways: RTK Signalweg