NOG Protein (AA 28-232)

Produktnummer ABIN7504481

Recombinant NOG-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für NOG Protein (AA 28-232) (ABIN7504481)

Target: NOG (Noggin (NOG))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Maus

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 28-232

Verwendungszweck: Mouse Noggin Protein

Sequenz: Gln28-Cys232

Produktmerkmale: Recombinant Mouse Noggin Protein is expressed from HEK293 without tag.It contains Gln28-Cys232.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 0.1 EU per μg by the LAL method.

Biological Activity Comment: Mouse Noggin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is 4-80 ng/mL in the presence of 50 ng/mL of recombinant Human BMP-4.,The purity of Mouse Noggin is greater than 95% as determined by SEC-HPLC.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS, 100 mM L-arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: NOG (Noggin (NOG))

Andere Bezeichnung: Noggin

Hintergrund: Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events.Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β-catenin, EGFR, ERK and Akt.

Molekulargewicht: 23.07 kDa. Due to glycosylation, the protein migrates to 28-33 kDa based on Bis-Tris PAGE result.

UniProt: P97466

Pathways: Stem Cell Maintenance, Tube Formation