ITCH Protein (AA 485-862)

Produktnummer ABIN7504453

Dieses Recombinant ITCH-Protein wird in Escherichia coli (E. coli) exprimiert.

Kurzübersicht für ITCH Protein (AA 485-862) (ABIN7504453)

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by Bis-Tris PAGE,> 90 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 485-862

Verwendungszweck: Human ITCH Protein

Sequenz: Arg485-Glu862

Produktmerkmale: Recombinant Human ITCH Protein is expressed from E.coli without tag.It contains Arg485-Glu862.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human ITCH on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human ITCH is greater than 90% as determined by SEC-HPLC.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS, 200 mM L-Arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))

Andere Bezeichnung: ITCH

Hintergrund: Itch or itchy E3 is a member of the homologous to E6-associated protein C-terminus (HECT)-type family of E3 ligases, with the protein-interacting WW-domains for the recruitment of substrate and the HECT domain for the transfer of ubiquitin to the substrate, and its deletion results in an itchy phenotype with constant skin scratching and multi-organ inflammation.

Molekulargewicht: 44.98 kDa same as Bis-Tris PAGE result.

NCBI Accession: NP_113671

Pathways: Activation of Innate immune Response, CXCR4-mediated Signaling Events