Cardiotrophin 1 Protein (CTF1)

Produktnummer ABIN7482110

Dieses Recombinant Cardiotrophin 1-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für Cardiotrophin 1 Protein (CTF1) (ABIN7482110)

Target: Cardiotrophin 1 (CTF1)

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: Escherichia coli (E. coli)

Applikation: Western Blotting (WB), Cell Culture (CC)

Reinheit: ≥98 % (HPLC)

Produktdetails

Verwendungszweck: Recombinant human Cardiotrophin-1 protein

Sequenz: SRREGSLEDP QTDSSVSLLP HLEAKIRQTH SLAHLLTKYA EQLLQEYVQL QGDPFGLPSF SPPRLPVAGL SAPAPSHAGL PVHERLRLDA AALAALPPLL DAVCRRQAEL NPRAPRLLRR LEDAARQARA LGAAVEALLA ALGAANRGPR AEPPAATASA ASATGVFPAK VLGLRVCGLY REWLSRTEGD LGQLLPGGSA

Spezifität: Toxin target: Leukemia Inhibitory Factor (LIF) receptor, ILST/gp130 receptors

Endotoxin-Niveau: <0.1 EU per 1 μg of the protein by the LAL method.

Biological Activity Comment: Cardiotrophin-1 is a member of the neuropoietic cytokine family which enhances the survival of ciliary and dopaminergic neurons through the interaction with the gp130 receptor. Cardiotrophin-1 induces cardiac myocyte hypertrophy and has been shown to promote the survival of both embryonic and neonatal rat ventricular muscle cells1-3.

Anwendungsinformationen

Applikationshinweise: Antigen Preadsorption Control: 10 - 100 nM

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Reconstitute in sterile, high-purity water at a concentration of at least 0.1 mg/mL. For long-term storage, aliquot the solution into small, single-use vials and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. For storage of dilute working solutions, we recommend adding a carrier protein (e.g., 0.1 % BSA) to your buffer to prevent material loss due to protein adsorption. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from sterile double distilled water (ddH2O).

Handhabung: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Lagerung: -20 °C

Informationen zur Lagerung: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Antigendetails

Target: Cardiotrophin 1 (CTF1)

Andere Bezeichnung: Cardiotrophin-1

Hintergrund: A recombinant human cytokine that promotes cardiac myocyte hypertrophy and survival, and acts as a neurotrophic factor via the gp130/LIFR receptor complex

Molekulargewicht: 21.1 kDa

UniProt: Q16619

Pathways: JAK-STAT Signalweg