GPRC5D Protein-VLP (AA 1-345) (Biotin)

Produktnummer ABIN7448170

VLP GPRC5D-Protein exprimiert in HEK-293 Cells.

Kurzübersicht für GPRC5D Protein-VLP (AA 1-345) (Biotin) (ABIN7448170)

Target: GPRC5D (G Protein-Coupled Receptor, Family C, Group 5, Member D (GPRC5D))

Protein-Typ: VLP

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Applikation: ELISA, Immunogen (Imm), Surface Plasmon Resonance (SPR), Functional Studies (Func)

Reinheit: > 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 1-345

Aufreinigungstag / Konjugat: Dieses GPRC5D Protein ist gelabelt mit Biotin.

Verwendungszweck: Biotinylated Human GPRC5D Protein-VLP

Sequenz: Met1-Val345

Produktmerkmale: Recombinant Biotinylated Human GPRC5D Protein-VLP is expressed from HEK293.It contains Met1-Val345.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μl by the LAL method.

Biological Activity Comment: The affinity constant of 0.30 nM as determined in SPR assay (Biacore T200).

Anwendungsinformationen

Applikationshinweise:

• Antibody Discovery: Immunization, Screening, Functional Characterization
• Affinity determination: ELISA, SPR
• In vivo pharmacokinetic analysis
• CMC method development
• CAR-T Positive Rate Detection
• Blood sample determination: ELISA

Kommentare:

Virus-like particles (VLPs) are formed from the outer capsid protein of a virus and are tiny nanoparticles formed by the automatic assembly of one or more capsid proteins. VLPs do not contain viral infectious genomes, so they are relatively safe during production operations. The SAMS™ protein engineering platform has been used to express a series of biotinylated, non-biotinylated, and fluorescently-labeled VLP-displayed antigens. They are suitable for SPR, ELISA, CAR-T positive rate detection, and other experimental scenarios.

Virus-Like Particles (VLPs) are highly immunogenic, meaning that they can elicit a strong immune response in the host. VLPs are recognized by the immune system and are taken up by antigen-presenting cells (APCs) such as dendritic cells. Once taken up by APCs, VLPs are processed and presented to T cells, which can trigger the activation of B cells to produce antibodies against the displayed antigen. Because VLPs resemble the structure and composition of native viruses, they are highly effective at inducing both humoral and cellular immune responses.

Generally, VLPs range in size from approximately 20 to 200 nanometers (nm). Compared to a cell-based immunization approach, their smaller size can optimize the immune response to target the specific antigen displayed on the surface of the engineered VLPs.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as 0.22μm filtered solution in PBS, 300 mM L-Arginine ( pH 7.4).

Lagerung: -80 °C

Informationen zur Lagerung: Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: GPRC5D (G Protein-Coupled Receptor, Family C, Group 5, Member D (GPRC5D))

Andere Bezeichnung: GPRC5D

Hintergrund: Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein-coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA.

Molekulargewicht: 39.6 kDa.

UniProt: Q9NZD1-1