Claudin 18.2 (AA 1-261) (Active) protein-VLP

Produktnummer ABIN7448160

Dieses VLP Claudin 18.2-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für Claudin 18.2 (AA 1-261) (Active) protein-VLP (ABIN7448160)

Target: Claudin 18.2

Protein-Typ: VLP

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Applikation: ELISA, Functional Studies (Func), Immunogen (Imm), Surface Plasmon Resonance (SPR)

Reinheit: > 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 1-261

Verwendungszweck: Human Claudin 18.2 VLP

Sequenz: Met1-Val261

Produktmerkmale: Human Claudin 18.2 VLP is expressed from HEK293. It contains Met1-Val261 (It may have cross reaction with anti-His antibody).

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human Claudin18.2 VLP captured on CM5 Chip via Anti-Claudin18.2 Antibody can bind Anti-Claudin18.2 Antibody with an affinity constant of 1.71 nM as determined in SPR assay (Biacore T200).,Immobilized Human Claudin 18.2 VLP at 5 ug/ml (100 ul/Well). Dose response curve for Anti-Claudin 18.2 Antibody, hFc Tag with the EC50 of 7.5 ng/ml determined by ELISA (QC Test).,The purity of Human Claudin 18.2 VLP is greater than 95% as determined by SEC-HPLC.

Anwendungsinformationen

Applikationshinweise:

• Antibody Discovery: Immunization, Screening, Functional Characterization
• Affinity determination: ELISA, SPR
• In vivo pharmacokinetic analysis
• CMC method development
• CAR-T Positive Rate Detection
• Blood sample determination: ELISA

Kommentare:

Virus-like particles (VLPs) are formed from the outer capsid protein of a virus and are tiny nanoparticles formed by the automatic assembly of one or more capsid proteins. VLPs do not contain viral infectious genomes, so they are relatively safe during production operations. The SAMS™ protein engineering platform has been used to express a series of biotinylated, non-biotinylated, and fluorescently-labeled VLP-displayed antigens. They are suitable for SPR, ELISA, CAR-T positive rate detection, and other experimental scenarios.

Virus-Like Particles (VLPs) are highly immunogenic, meaning that they can elicit a strong immune response in the host. VLPs are recognized by the immune system and are taken up by antigen-presenting cells (APCs) such as dendritic cells. Once taken up by APCs, VLPs are processed and presented to T cells, which can trigger the activation of B cells to produce antibodies against the displayed antigen. Because VLPs resemble the structure and composition of native viruses, they are highly effective at inducing both humoral and cellular immune responses.

Generally, VLPs range in size from approximately 20 to 200 nanometers (nm). Compared to a cell-based immunization approach, their smaller size can optimize the immune response to target the specific antigen displayed on the surface of the engineered VLPs.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Supplied as 0.22 μm filtered solution in PBS, 300 mM L-arginine ( pH 7.4). Notice: If you need it for immunization, water-soluble adjuvant is recommended.

Lagerung: -80 °C

Informationen zur Lagerung: Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: Claudin 18.2

Hintergrund: Claudin18 (CLDN18) belongs to the large claudin family of proteins, which form tight junction strands in epithelial cells. CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants, CLDN18.1 and CLDN18.2. Isoform 2 (Claudin 18.2) is abundant in gastric tumors.

Molekulargewicht: 29 kDa.

UniProt: P56856