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VEGF 165 (AA 27-191) Protein

Recombinant -Protein exprimiert in HEK-293 Cells.
Produktnummer ABIN7275824

Kurzübersicht für VEGF 165 (AA 27-191) Protein (ABIN7275824)

Target

VEGF 165

Protein-Typ

Recombinant

Spezies

  • 19
  • 2
  • 1
Human

Quelle

  • 10
  • 6
  • 2
  • 1
  • 1
  • 1
  • 1
HEK-293 Cells

Reinheit

> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
  • Proteineigenschaft

    AA 27-191

    Verwendungszweck

    Human VEGF165 Protein

    Sequenz

    Ala27-Arg191

    Produktmerkmale

    Recombinant Human VEGF165 Protein is expressed from HEK293 without tag.It contains Ala27-Arg191.

    Sterilität

    0.22 μm filtered

    Endotoxin-Niveau

    Less than 0.05 EU per μg by the LAL method.

    Biological Activity Comment

    Immobilized Human VEGF165 at 1μg/ml (100μl/well) on the plate. Dose response curve for Human VEGFR2, mFc Tag with the EC50 of 74.2ng/ml determined by ELISA. See testing image for detail.
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  • Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

    Buffer

    Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

    Lagerung

    -20 °C,-80 °C

    Informationen zur Lagerung

    -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

    Haltbarkeit

    12 months
  • Target

    VEGF 165

    Andere Bezeichnung

    VEGF165

    Hintergrund

    Human papillomavirus (HPV) infection is an established risk factor for cervical carcinogenesis. VEGF165 was significantly higher, whereas VEGFC and VEGFD were significantly lower in malignant cervical carcinoma tissues as compared to normal cervix tissues. Expression levels of VEGF121 and VEGFC were significantly associated with type of tumor growth while VEGF165 was significantly associated with lymph node metastasis.

    Molekulargewicht

    19.2 kDa. Due to glycosylation, the protein migrates to 20-30 kDa based on Tris-Bis PAGE result.

    UniProt

    P15692-4
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