LILRB3 Protein (AA 24-443) (Biotin,His-Avi Tag)

Produktnummer ABIN7275190

Dieses Recombinant LILRB3-Protein wird in HEK-293 Cells exprimiert.

Kurzübersicht für LILRB3 Protein (AA 24-443) (Biotin,His-Avi Tag) (ABIN7275190)

Target: LILRB3 (Leukocyte Immunoglobulin-Like Receptor B3 (LILRB3))

Protein-Typ: Recombinant

Biologische Aktivität: Active

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 24-443

Aufreinigungstag / Konjugat: Dieses LILRB3 Protein ist gelabelt mit Biotin,His-Avi Tag.

Verwendungszweck: Biotinylated Human LILRB3/CD85a/ILT5 Protein

Sequenz: Gly24-Glu443

Produktmerkmale: Recombinant Biotinylated Human LILRB3/CD85a/ILT5 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly24-Glu443.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human LILRB3, His Tag at 2ug/ml (100ul/well) on the streptavidin precoated plate (5ug/ml). Dose response curve for Anti-LILRB3 Antibody, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA.,Biotinylated Human LILRB3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human LILRB3 is greater than 95% as determined by SEC-HPLC.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: LILRB3 (Leukocyte Immunoglobulin-Like Receptor B3 (LILRB3))

Andere Bezeichnung: LILRB3

Hintergrund: Leukocyte immunoglobulin-like receptor subfamily B (LILRB3), also known as ILT5, LIR3, and CD85a, is an immunoglobulin superfamily member that is involved in immune regulation. Subfamily B members have cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP-1.LILRB3 may act as receptor for class I MHC antigens. Becomes activated upon coligation of LILRB3 and immune receptors, such as FCGR2B and the B-cell receptor.

Molekulargewicht: 50 kDa. Due to glycosylation, the protein migrates to 60-72 kDa based on Bis-Tris PAGE result.

UniProt: O75022