IL-7 Protein (AA 26-177)
Kurzübersicht für IL-7 Protein (AA 26-177) (ABIN7274963)
Target
Alle IL-7 (IL7) Proteine anzeigenProtein-Typ
Spezies
Quelle
Reinheit
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Proteineigenschaft
- AA 26-177
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Verwendungszweck
- Human IL-7 Protein
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Sequenz
- Asp26-His177
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Produktmerkmale
- Recombinant Human IL-7 Protein is expressed from E.coli without tag.It contains Asp26-His177.
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Sterilität
- 0.22 μm filtered
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Endotoxin-Niveau
- Less than 0.1 EU per μg by the LAL method.
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Biological Activity Comment
- Immobilized Human IL-7 at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-7R alpha, hFc Tag with the EC50 of 16.2ng/ml determined by ELISA (QC tested). The affinity constant of 7.15 nM as determined in SPR assay (Biacore T200). See testing image for detail.
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Interleukin 7 (IL7) (AA 26-154) (Active) protein (His tag)
IL7 Spezies: Maus Wirt: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Interleukin 7 (IL7) (AA 26-177) (Active) protein (His tag)IL7 Spezies: Human Wirt: Escherichia coli (E. coli) > 95 % AcA, CC Active
Interleukin 7 (IL7) (AA 26-154) protein (His tag)IL7 Spezies: Ratte Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 7 (IL7) (AA 26-177) protein (His-IF2DI Tag)IL7 Spezies: Human Wirt: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 7 (IL7) (Active) protein (His tag)IL7 Spezies: Maus Wirt: Human Cells Recombinant > 95 % as determined by SDS-PAGE Active
Interleukin 7 (IL7) (AA 26-177) protein (GST tag)IL7 Spezies: Human Wirt: Wheat germ Recombinant WB, ELISA, AA, AP
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Lyophilized
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Rekonstitution
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
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Buffer
- Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
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Lagerung
- -20 °C,-80 °C
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Informationen zur Lagerung
- -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
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Haltbarkeit
- 12 months
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- IL-7 (IL7) (Interleukin 7 (IL7))
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Andere Bezeichnung
- IL-7
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Hintergrund
- Interleukin-7 (IL7) plays a nonredundant role in T cell survival and homeostasis, which is illustrated in the severe T cell lymphopenia of IL7-deficient mice, or demonstrated in animals or humans that lack expression of either the IL7Rα or γ c chain, the two subunits that constitute the functional IL7 receptor.
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Molekulargewicht
- 17.5 kDa same as Tris-Bis PAGE result.
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UniProt
- P13232
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Pathways
- JAK-STAT Signalweg
Target
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