GFRAL Protein (AA 20-350) (His-Avi Tag,Biotin)

Produktnummer ABIN7274740

Produktdetails

Target: GFRAL (GDNF Family Receptor alpha Like (GFRAL))

Protein-Typ: Recombinant

Proteineigenschaft: AA 20-350

Spezies: Maus

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses GFRAL Protein ist gelabelt mit His-Avi Tag,Biotin.

Verwendungszweck: Biotinylated Mouse GFRAL/GFR alpha-like Protein

Sequenz: Gln20-Glu350

Produktmerkmale: Recombinant Biotinylated Mouse GFRAL/GFR alpha-like Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus.It contains Gln20-Glu350.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Mouse GFRAL, His Tag at 0.5μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Mouse GDF15, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: GFRAL (GDNF Family Receptor alpha Like (GFRAL))

Andere Bezeichnung: GFRAL (GFRAL Produkte)

Hintergrund: GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.

Molekulargewicht: 40.1 kDa. Due to glycosylation, the protein migrates to 48-60 kDa based on Tris-Bis PAGE result.