FGF2 Protein (AA 143-288)

Produktnummer ABIN7274613

Dieses Recombinant FGF2-Protein wird in Escherichia coli (E. coli) produziert.

Kurzübersicht für FGF2 Protein (AA 143-288) (ABIN7274613)

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Protein-Typ: Recombinant

Spezies: Human

Quelle: Escherichia coli (E. coli)

Reinheit: > 95 % as determined by Tris-Bis PAGE

Produktdetails

Proteineigenschaft: AA 143-288

Verwendungszweck: Human FGF basic Protein

Sequenz: Pro143-Ser288

Produktmerkmale: Recombinant Human FGF basic Protein is expressed from E.coli without tag., It contains Pro143-Ser288.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 0.02 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human FGF basic at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human Glypican 1, His Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Andere Bezeichnung: FGF basic

Hintergrund: FGF basic is a member of the FGF family of at least 23 related mitogenic proteins which show 35-60 % amino acid conservation. FGF acidic and basic, unlike the other members of the family, lack signal peptides and are apparently secreted by mechanisms other than the classical protein secretion pathway.

Molekulargewicht: 16.5 kDa same as Tris-Bis PAGE result.

UniProt: P09038-4

Pathways: RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteine