EPH Receptor B2 Protein (EPHB2) (AA 19-543) (His tag)

Produktnummer ABIN7274553

Dieses Recombinant EPH Receptor B2-Protein wird in HEK-293 Cells produziert.

Kurzübersicht für EPH Receptor B2 Protein (EPHB2) (AA 19-543) (His tag) (ABIN7274553)

Target: EPH Receptor B2 (EPHB2)

Protein-Typ: Recombinant

Spezies: Human

Quelle: HEK-293 Cells

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Produktdetails

Proteineigenschaft: AA 19-543

Aufreinigungstag / Konjugat: Dieses EPH Receptor B2 Protein ist gelabelt mit His tag.

Verwendungszweck: Human EPHB2 Protein

Sequenz: Val19-Leu543

Produktmerkmale: Recombinant Human EPHB2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val19-Leu543.

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human EPHB2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-EPHB2 Antibody, hFc Tag with the EC50 of 14.9ng/ml determined by ELISA. See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: EPH Receptor B2 (EPHB2)

Andere Bezeichnung: EPHB2

Hintergrund: EphB2, a receptor tyrosine kinase for ephrin ligands, is overexpressed in various cancers and plays an important role in tumor progression. EPHB2 promotes endothelial-mesenchymal transition (EMT) and elicits associated pathologic characteristics of glioblastoma multiforme (GBM) such as invasion and migration. EPHB2 is epigenetically overexpressed in hypoxia, a condition highly prevalent in malignancy. Furthermore, HIF-2α is required for EPHB2 stabilization by hypoxia.

Molekulargewicht: 59.2 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.

UniProt: P29323-1

Pathways: RTK Signalweg, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteine