CD226 Protein (CD226) (AA 19-247) (Fc Tag)

Produktnummer ABIN7274457

Produktdetails

Target: CD226

Protein-Typ: Recombinant

Proteineigenschaft: AA 19-247

Spezies: Human

Quelle: HEK-293 Cells

Aufreinigungstag / Konjugat: Dieses CD226 Protein ist gelabelt mit Fc Tag.

Verwendungszweck: Human DNAM-1/CD226 Protein

Sequenz: Glu19-Asn247

Produktmerkmale: Recombinant Human DNAM-1/CD226 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu19-Asn247.

Reinheit: > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC

Sterilität: 0.22 μm filtered

Endotoxin-Niveau: Less than 1EU per μg by the LAL method.

Biological Activity Comment: Immobilized Biotinylated Human CD155, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 4.8μg/ml determined by ELISA. The affinity constant of 0.234 μM as determined in SPR assay (Biacore T200). See testing image for detail.

Anwendungsinformationen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.

Buffer: Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Haltbarkeit: 12 months

Antigendetails

Target: CD226

Andere Bezeichnung: DNAM-1 (CD226 Produkte)

Hintergrund: DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.

Molekulargewicht: 52.8 kDa. Due to glycosylation, the protein migrates to 70-100 kDa based on Tris-Bis PAGE result.

UniProt: Q15762

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints